#Gms stain professional
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Provide sufficient details of any financial or non-financial competing interests to enable users to assess whether your comments might lead a reasonable person to question your impartiality. Sensitivity of both methods can be increased by preparing two slides from different levels in the paraffin block.
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Four out of 30 cases were negative with original PAS stain, but stained positive both with new PAS and GMS.ĬONCLUSIONS: PAS and GMS stains are quantitatively similar. Repetition of GMS stain on these three cases (cutting in a deeper level in the paraffin block) resulted in positive results. The difference between the two stains is statistically insignificant. RESULTS: Twenty-two cases were positive with PAS, staining three cases more than GMS, which stained only 19 cases. The results were also compared with the original PAS stain. The presence or absence of fungal organisms was evaluated. METHODS: We performed both PAS and GMS stains on 30 clinically suspected onychomycosis cases. We conducted a similar study with the addition of repeating PAS stain and compared the newly stained PAS and GMS slides in tandem. without repeating PAS) with newly stained Grocott methenamine-silver (GMS) slide, D'Hue et al.
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By comparing originally stained PAS slides (i.e. Periodic acid-Schiff (PAS) stain has been considered the most sensitive method for diagnosis. BACKGROUND: Onychomycosis is the most common nail disease in adults.